Golgi 抗体
适用: 人
IHC (p)
宿主: 兔
Monoclonal
GLG1-2829R
unconjugated
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- 抗原
- Golgi
- 适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Purified
- 纯度
- Protein A affinity chromatography
- 免疫原
- SU-DHL-1 large cell lymphoma cells were used as the immunogen for this recombinant Golgi antibody.
- 克隆位点
- GLG1-2829R
- 亚型
- IgG kappa
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anti-Golgi antibody
适用: 人 IHC (p), FACS, IF, ICC, WB 宿主: 小鼠 Monoclonal AE-6 unconjugated
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- 应用备注
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The optimal dilution of the recombinant Golgi antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) - 限制
- 仅限研究用
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- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the recombinant Golgi antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Golgi
- 背景
- This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.
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