CD99 抗体
Quick Overview for CD99 抗体 (ABIN6251917)
抗原
See all CD99 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
纯化方法
- PEG precipitation
-
免疫原
- Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.
-
亚型
- IgM kappa
-
-
anti-CD99 (CD99) (AA 1-185) antibody
CD99 适用: 人 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7E11C4 unconjugated
anti-CD99 (CD99) (AA 1-185) antibodyCD99 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1B10E12 unconjugated
anti-CD99 (CD99) (AA 23-122) antibodyCD99 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-CD99 (CD99) (AA 23-185) antibodyCD99 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) (AA 27-161) antibodyCD99 适用: 小鼠 FACS, WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) (AA 26-160) antibodyCD99 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD99 (CD99) antibodyCD99 适用: 人 FACS, MS 宿主: 小鼠 Monoclonal 3B2-TA8 unconjugated
anti-CD99 (CD99) antibodyCD99 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5C8 unconjugated
anti-CD99 (CD99) antibody (PE)CD99 适用: 人 FACS 宿主: 小鼠 Monoclonal 3B2-TA8 PE
anti-CD99 (CD99) antibodyCD99 适用: 人 FACS, WB, IHC, ELISA 宿主: 兔 Monoclonal 9A5 unconjugated Recombinant Antibody
-
-
应用备注
-
Western blot: 1-2 μg/mL
Flow Cytometry: 5-10 μL/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the CD99 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.2 mg/mL
-
缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
-
- CD99
-
别名
- CD99
-
背景
- CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]
-
基因ID
- 4267
-
UniProt
- P14209
抗原
-
