TFF1 抗体 (C-Term)
Quick Overview for TFF1 抗体 (C-Term) (ABIN6251839)
抗原
See all TFF1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (57-84) from the C-terminus of human Estrogen inducible protein pS2 was used as the immunogen for this antibody.
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亚型
- IgG1 kappa
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anti-Trefoil Factor 1 (TFF1) antibody
TFF1 适用: 人 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal TFF1-2133 unconjugated
anti-Trefoil Factor 1 (TFF1) (AA 25-84), (pSer2) antibodyTFF1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Trefoil Factor 1 (TFF1) antibodyTFF1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Trefoil Factor 1 (TFF1) (AA 1-87) antibodyTFF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Trefoil Factor 1 (TFF1) (AA 1-81) antibodyTFF1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Trefoil Factor 1 (TFF1) (AA 1-84) antibodyTFF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3H5 unconjugated
anti-Trefoil Factor 1 (TFF1) (AA 57-84) antibodyTFF1 适用: 人, Cynomolgus IHC, StM 宿主: 小鼠 Monoclonal GE2-same as R47-94 unconjugated
anti-Trefoil Factor 1 (TFF1) (AA 1-84) antibodyTFF1 适用: 人 WB, IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Trefoil Factor 1 (TFF1) (AA 1-88) antibodyTFF1 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-482M unconjugated
anti-Trefoil Factor 1 (TFF1) (AA 25-84) antibodyTFF1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues REQUIRES digestion with Pronase E at 1 mg/mL in PBS for 15 min at 37 °C. Heat Induced Epitope Retrieval is not suitable for this antibody.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- TFF1 (Trefoil Factor 1 (TFF1))
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别名
- Estrogen Inducible Protein pS2
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背景
- This antibody recognizes a polypeptide of ~6.5 kDa, identified as Estrogen Inducible Protein pS2. The antibody's epitope is localized between amino acids 57-84. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. Estrogen Inducible Protein pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60 % of breast carcinomas are positive for estrogen inducible protein pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that estrogen inducible protein pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.
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基因ID
- 7031
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UniProt
- P04155
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途径
- EGFR Signaling Pathway
抗原
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