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Myeloid Cell Marker 抗体

The 小鼠 单克隆 anti-Myeloid Cell Marker antibody is suitable to detect Myeloid Cell Marker in samples from 人. It has been validated for FACS, IF 和 IHC.
产品编号 ABIN6251824
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Myeloid Cell Marker 抗体 (ABIN6251824)

抗原

Myeloid Cell Marker

适用

宿主

  • 3
小鼠

克隆类型

  • 3
单克隆

标记

  • 3
非结合性

应用范围

  • 2
  • 2
  • 2
  • 1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

克隆位点

BM-1
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Human peripheral blood mononuclear cells were used as the immunogen for this Myeloid cell marker antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells

    IF: 1-2 μg/mL

    IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Myeloid cell marker antibody to be titered up or down for optimal performance.

    1. No special pretreatment is required for staining of formalin/paraffin tissues.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    Myeloid Cell Marker

    背景

    This antibody recognizes a 183  kDa protein with DNA-binding characteristics, which is identified as a myeloid cell specific antigen. Clone BM-1 antibody reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. This type of marker is useful in the identification of different levels of cellular differentiation. BM-1 and BM-2 antibodies react with early precursor and mature forms of human myeloid cells. This mAb is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of the marker protein is not clear, although it has been proposed that it may play a role in cell differentiation.
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