SUMO2/3 抗体
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北京 101111
Quick Overview for SUMO2/3 抗体 (ABIN6251816)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human protein was used as the immunogen for this SUMO-2/3 antibody.
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亚型
- IgG1 kappa
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anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (full length) antibody
SUMO2/3 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, IHC (fro) 宿主: 大鼠 Monoclonal 3H12 unconjugated
anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (AA 49-81) antibodySUMO2/3 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (AA 53-84), (C-Term) antibodySUMO2/3 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB15398 unconjugated
anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (AA 53-84) antibodySUMO2/3 适用: 人 WB, IF, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (AA 38-64) antibodySUMO2/3 适用: 人 WB 宿主: 兔 Polyclonal RB3377 unconjugated
anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (cleaved), (Gly93) antibodySUMO2/3 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the SUMO-2/3 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))
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别名
- SUMO-2/3
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背景
- This mAb reacts with both SUMO-2 and SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer s disease.
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基因ID
- 6613
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UniProt
- P61956
抗原
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