Smooth Muscle Actin 抗体
Quick Overview for Smooth Muscle Actin 抗体 (ABIN6251798)
抗原
See all Smooth Muscle Actin (ACTA2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- A 10 amino acid peptide from the alpha smooth muscle isoform of actin was used as the immunogen for this Smooth Muscle Actin antibody.
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亚型
- IgG2a kappa
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anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody
Verified ACTA2 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyACTA2 适用: 人, 大鼠, 小鼠, Cow WB, IHC, IF, ELISA, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyKO Validated ACTA2 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyACTA2 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal 1A4-ACTA2-791 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-124) antibodyACTA2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 2-124) antibodyACTA2 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 5C1H11 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibodyACTA2 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibodyACTA2 适用: 人, 大鼠, 小鼠, 小鸡, 兔, 山羊 WB, IHC, ELISA, IHC (p), ICC, IHC (fro), IEM 宿主: 小鼠 Monoclonal alpha-SM1-1A4 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-377) antibodyACTA2 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 4A8-2H3 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibodyACTA2 适用: 人, 猴 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4F4 unconjugated
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应用备注
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Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The optimal dilution of the Smooth Muscle Actin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
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别名
- Smooth Muscle Actin
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背景
- Actin is a major component of the cytoskeleton and is present in most cell types. This mAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This mAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
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基因ID
- 59
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UniProt
- P62736
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途径
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
抗原
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