FTL 抗体 (AA 38-165) (CF®647)
Quick Overview for FTL 抗体 (AA 38-165) (CF®647) (ABIN6191672)
抗原
See all FTL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 38-165
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原理
- Mouse Monoclonal anti-Ferritin, Light chain (FTL/1389), CF647 Conjugate
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产品特性
- Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human FTL protein fragment (aa 38-165) (exact sequence is proprietary)
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亚型
- IgG1
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应用备注
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Immunohistology (formalin) 0.1-0.2 μg/mL
- Flow cytometry 0.1-0.2 μg/million cells Immunofluorescence 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Optimal dilution for a specific application should be determined by user
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说明
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HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- FTL (Ferritin, Light Polypeptide (FTL))
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别名
- Ferritin, Light chain
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背景
- Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, FTL, LFTD, NBIA3
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分子量
- 19-25 kDa
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基因ID
- 2512, 433670
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UniProt
- P02792
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途径
- Transition Metal Ion Homeostasis
抗原
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