Elastase 3B 抗体 (AA 82-238)
Quick Overview for Elastase 3B 抗体 (AA 82-238) (ABIN6191585)
抗原
See all Elastase 3B (ELA3B) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 82-238
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原理
- Mouse Monoclonal anti-CELA3B / ELA3B (CELA3B/1811), Purified, with BSA
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产品特性
- This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
- Purified
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免疫原
- Recombinant human CELA3B protein fragment (aa82-238) (exact sequence is proprietary)
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亚型
- IgG2b kappa
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anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibody
ELA3B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CELA3B-1257 unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyELA3B 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CELA3B-1757 unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyELA3B 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-410M unconjugated Recombinant Antibody
anti-Elastase 3B, Pancreatic (ELA3B) (AA 28-267) antibodyELA3B 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 19-270) antibodyELA3B 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3H3 unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyELA3B 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rCELA3B-1811 unconjugated Recombinant Antibody
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyELA3B 适用: 人 IHC, StM 宿主: 兔 Monoclonal CELA3B-2809R unconjugated Recombinant Antibody
anti-Elastase 3B, Pancreatic (ELA3B) (AA 82-238) antibodyELA3B 适用: 人, 小鼠, 大鼠 ELISA, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal CELA3B-1257 unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 29-270) antibodyELA3B 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Elastase 3B, Pancreatic (ELA3B) (AA 19-270) antibodyELA3B 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2H6 unconjugated
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应用备注
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ELISA 2-4 μg/mL for coating (order Ab without BSA)
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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Panc-28 or PANC1 cells. Pancreas
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 200 μg/mL
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缓冲液
- PBS/0.05 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
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别名
- CELA3B / ELA3B
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背景
- Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E
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分子量
- 17 kDa
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基因ID
- 23436, 728752
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UniProt
- P08861
抗原
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