Nuclear Antigen 抗体 (CF®555)
Quick Overview for Nuclear Antigen 抗体 (CF®555) (ABIN6190800)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Human Nuclear Antigen (235-1), CF555 Conjugate
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产品特性
- This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Nuclei of human myeloid leukemia biopsy cells
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亚型
- IgG1 kappa
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anti-Nuclear Antigen antibody
适用: 人, 大鼠, 小鼠 IF, ICC, FACS, IHC 宿主: 小鼠 Monoclonal NM106 unconjugated
anti-Nuclear Antigen antibody适用: 人, 灵长类 IF, ICC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 235-1 unconjugated
anti-Nuclear Antigen antibody适用: 人, 大鼠, 小鼠 IF, ICC, IHC (p) 宿主: 小鼠 Monoclonal NUCM1-1 unconjugated
anti-Nuclear Antigen antibody适用: 人, Non-Human Primate IF, ICC, FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal 235-1 unconjugated
anti-Nuclear Antigen antibody适用: 人, 灵长类 IF, ICC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 235-1 unconjugated
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应用备注
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Immunohistology Frozen 0.5-1.0 μg/mL
- Immunocytochemistry acetone-fixed cells 0.25-0.5 μg/mL
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunoprecipitation 0.5-1 μg/500 μg protein lysate
- Does not react with mouse, rat or chicken, others not known
- Optimal dilution for a specific application should be determined by user
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说明
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All human cells. Tonsil
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Nuclear Antigen
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背景
- HNA, HuNu
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分子量
- 70 kDa & 80 kDa
抗原
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