pan Keratin 抗体 (CF®647)
Quick Overview for pan Keratin 抗体 (CF®647) (ABIN6190228)
抗原
See all pan Keratin (panKRT) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Cytokeratin, pan (AE-1 / AE-3), CF647 Conjugate
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产品特性
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human epidermal keratin
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亚型
- IgG
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anti-pan Keratin (panKRT) antibody
panKRT 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7H8C4 unconjugated
anti-pan Keratin (panKRT) (AA 331-430) antibodypanKRT 适用: 人, 大鼠 IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-pan Keratin (panKRT) (pan) antibodypanKRT 适用: 人, 大鼠, 小鼠 WB, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-000R unconjugated Recombinant Antibody
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人, 大鼠, 小鼠, Cow, 猴, 犬, 兔, 小鸡 IF, FACS, WB, IHC (p) 宿主: 小鼠 Monoclonal AE1-AE3 unconjugated
anti-pan Keratin (panKRT) antibodyGMP Grade panKRT 适用: 人 FACS 宿主: 小鼠 Monoclonal MCK457 unconjugated
anti-pan Keratin (panKRT) antibody (iFluor™488)GMP Grade panKRT 适用: 人 FACS 宿主: 小鼠 Monoclonal MCK457 iFluor™488
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人, 大鼠, 小鼠, Cow, Pig, Frog, 豚鼠, 山羊, Marmoset IF, FACS, WB, IHC, StM 宿主: 小鼠 Monoclonal SPM583 unconjugated
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人, 大鼠 IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal KRTL-1077-KRTH-1076 unconjugated
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应用备注
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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Skin, Adeno- or Squamous carcinomas
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- pan Keratin (panKRT)
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别名
- Cytokeratin, pan
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背景
- K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39
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分子量
- 40-67 kDa
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基因ID
- 374454, 51350
抗原
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