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pan Keratin 抗体 (CF®594)

This CF®594-conjugated 小鼠 单克隆 anti-pan Keratin antibody (Clone C11) specifically detects pan Keratin in IF, FACS, WB 和 IHC (f). The antibody is reactive with 人, 大鼠, 小鼠, Cow, Pig, Frog, 豚鼠, 山羊 和 Marmoset samples.
产品编号 ABIN6190168
发货至: 中国
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北京 101111
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Quick Overview for pan Keratin 抗体 (CF®594) (ABIN6190168)

抗原

See all pan Keratin (panKRT) 抗体
pan Keratin (panKRT)

适用

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人, 大鼠, 小鼠, Cow, Pig, Frog, 豚鼠, 山羊, Marmoset

宿主

  • 95
  • 70
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小鼠

克隆类型

  • 130
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单克隆

标记

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This pan Keratin antibody is conjugated to CF®594

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

C11
  • 原理

    Mouse Monoclonal anti-Cytokeratin, multi (C11), CF594 Conjugate

    产品特性

    This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Keratin-enriched preparation from cultured human A431

    亚型

    IgG1
  • 应用备注

    Immunohistology formalin-fixed 0.5-1.0 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 0.5-1.0 μg/mL
    • Western blotting 0.5-1.0 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    A431 cells, Skin, Colon carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    pan Keratin (panKRT)

    别名

    Cytokeratin, multi

    背景

    K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

    分子量

    Multiple

    基因ID

    3851, 3852, 3853
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