pan Keratin 抗体 (CF®647)
Quick Overview for pan Keratin 抗体 (CF®647) (ABIN6190152)
抗原
See all pan Keratin (panKRT) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Cytokeratin, multi (C11), CF647 Conjugate
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产品特性
- This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Keratin-enriched preparation from cultured human A431
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亚型
- IgG1
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应用备注
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Immunohistology formalin-fixed 0.5-1.0 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Western blotting 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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A431 cells, Skin, Colon carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- pan Keratin (panKRT)
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别名
- Cytokeratin, multi
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背景
- K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39
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分子量
- Multiple
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基因ID
- 3851, 3852, 3853
抗原
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