TYR 抗体 (CF®405S)
Quick Overview for TYR 抗体 (CF®405S) (ABIN6189726)
抗原
See all TYR 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Tyrosinase (OCA1/812), CF405S Conjugate
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产品特性
- This antibody recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human TYR protein
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亚型
- IgG2a kappa
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anti-Tyrosinase (TYR) (AA 1-377) antibody
TYR 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2A2-F4 unconjugated
anti-Tyrosinase (TYR) (AA 20-340) antibodyTYR 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) (AA 486-513) antibodyTYR 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) (AA 1-377) antibodyTYR 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) antibodyTYR 适用: 人, 小鼠, 猴 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) antibodyTYR 适用: 人 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal T311 unconjugated
anti-Tyrosinase (TYR) (C-Term) antibodyTYR 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) (AA 486-513), (C-Term) antibodyTYR 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB19155 unconjugated
anti-Tyrosinase (TYR) (C-Term) antibodyTYR 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) antibodyTYR 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal T311 unconjugated
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应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, or 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- ELISA For coating, order Ab without BSA
- Optimal dilution for a specific application should be determined by user
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说明
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SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- TYR (Tyrosinase (TYR))
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别名
- Tyrosinase
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背景
- ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR
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分子量
- 70-80 kDa
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基因ID
- 7299, 503555
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UniProt
- P14679
抗原
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