ETS1 抗体 (AA 137-230)
Quick Overview for ETS1 抗体 (AA 137-230) (ABIN6187635)
抗原
See all ETS1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 137-230
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原理
- Mouse Monoclonal anti-ETS1-/ (ETS1/1282), Purified, BSA-free
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产品特性
- ETS1 proto-oncogene is an important transcription factor that plays a role in cell proliferation and differentiation. ETS1 is related to the growth of carcinoma cells by its regulation of the transcription of matrix metalloproteinases and urokinase-type plasminogen activator. The processes of tumor invasion and metastasis depend on the increased proteolytic activity of the invading tumor cells that may involve matrix metalloproteinases, cathepsins B and D and plasminogen activator in the metastatic cascade. ETS1 is preferentially expressed in lymphoid cells, where it is essential for the maintenance of the normal pool of resting T and B cells. ETS1 expression level and distribution are differentially controlled in resting, activated and apoptotic lymphocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
- Purified
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免疫原
- Recombinant human ETS1 fragment (aa137-230) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
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Flow cytometry 0.5-1 μg/million cells in 0.1 mL
- Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Western blotting 1-2 μg/mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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HepG2 and HeLa cells. Tonsil, Skin, Pancreas, Kidney and Lymphoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS (no BSA, no azide)
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储存液
- Azide free
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- ETS1 (V-Ets erythroblastosis Virus E26 Oncogene Homolog 1 (Avian) (ETS1))
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别名
- ETS1-
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背景
- Avian erythroblastosis virus E26 (v ets) oncogene homolog 1, ETS1, EWSR2, p54, v-ets erythroblastosis virus E26 oncogene homolog 1
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分子量
- 54 kDa
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基因ID
- 2113, 369438
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UniProt
- P14921
抗原
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