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CD81 抗体 (CF®488A)

This anti-CD81 antibody is a 小鼠 单克隆 antibody detecting CD81 in FACS, WB, IHC, IF 和 Func. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN6187164
发货至: 中国

Quick Overview for CD81 抗体 (CF®488A) (ABIN6187164)

抗原

See all CD81 抗体
CD81

适用

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人, 小鼠, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD81 antibody is conjugated to CF®488A

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func)

克隆位点

1-3-3-22
  • 原理

    Mouse Monoclonal anti-CD81 / TAPA-1 (1.3.3.22), CF488A Conjugate

    产品特性

    This antibody This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI, Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    B-Cell line derived from a Burkitt lymphoma

    亚型

    IgG1 kappa
  • 应用备注

    For functional studies, order Ab without azide
    • Immunohistology (formalin) 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, and human lymphocytes. Tonsil or Liver.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    CD81

    别名

    CD81 / TAPA-1

    背景

    CD81, Target of the antiproliferative antibody 1 (TAPA-1), Tetraspanin-28, Tspan-28

    分子量

    26 kDa

    基因ID

    975, 54457

    UniProt

    P60033

    途径

    Inositol Metabolic Process, Hepatitis C
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