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HTR2C 抗体 (AA 439-460)

This anti-HTR2C antibody is a 兔 多克隆 antibody detecting HTR2C in WB, IHC, IF, ICC 和 IEM. Suitable for 小鼠 和 大鼠. This Primary Antibody has been cited in 4+ publications.
产品编号 ABIN617931
发货至: 中国

Quick Overview for HTR2C 抗体 (AA 439-460) (ABIN617931)

抗原

See all HTR2C 抗体
HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

适用

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小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This HTR2C antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)
  • 抗原表位

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    AA 439-460

    交叉反应

    人, 小鼠, 大鼠

    产品特性

    The 5-HT 2C Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 439-460 of the rat 5-HT2C receptor coupled to KLH and bovine thyroglobulin. The 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are /500 - 1/1000 in PBS - Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.

    纯化方法

    Affinity Purified

    免疫原

    Rat 5-HT2C receptor (439-460)

    亚型

    IgG
  • 限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Contains ≤ 0.09 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.
  • Zhang, Li, Xin, Lao, Ren, Berman, Tan, Zhang: "Involvement of spinal serotonin receptors in electroacupuncture anti-hyperalgesia in an inflammatory pain rat model." in: Neurochemical research, Vol. 36, Issue 10, pp. 1785-92, (2011) (PubMed).

    Coulon, Kanyshkova, Broicher, Munsch, Wettschureck, Seidenbecher, Meuth, Offermanns, Pape, Budde: "Activity Modes in Thalamocortical Relay Neurons are Modulated by G(q)/G(11) Family G-proteins - Serotonergic and Glutamatergic Signaling." in: Frontiers in cellular neuroscience, Vol. 4, pp. 132, (2011) (PubMed).

    Yang, Mahaffey, Bérubé, Maddatu, Cox, Frankel: "Complex seizure disorder caused by Brunol4 deficiency in mice." in: PLoS genetics, Vol. 3, Issue 7, pp. e124, (2007) (PubMed).

    Holmes: "5-Hydroxytryptamine2C receptors on pudendal motoneurons innervating the external anal sphincter." in: Brain research, Vol. 1057, Issue 1-2, pp. 65-71, (2005) (PubMed).

  • 抗原

    HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

    别名

    Serotonin Receptor 2C (HTR2C)

    背景

    Other Names:
    5-hydroxytryptamine receptor 2C,5-HT2C, 5HT-1C, 5-HTR2C,5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled

    基因ID

    25187

    途径

    Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour
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