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Cytokeratin 18 抗体

This anti-Cytokeratin 18 antibody is a 小鼠 单克隆 antibody detecting Cytokeratin 18 in IF, FACS 和 IHC (f). Suitable for 人, 小鼠, 大鼠, 犬, Pig, Cat, Cow, Hamster, 绵羊, 马, 雪貂, 山羊, Marmoset 和 猴.
产品编号 ABIN6179199
发货至: 中国

Quick Overview for Cytokeratin 18 抗体 (ABIN6179199)

抗原

See all Cytokeratin 18 (KRT18) 抗体
Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

适用

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人, 小鼠, 大鼠, 犬, Pig, Cat, Cow, Hamster, 绵羊, 马, 雪貂, 山羊, Marmoset, 猴

宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 18 antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

C-04
  • 原理

    Mouse Monoclonal anti-Cytokeratin 18 (C-04), Purified, BSA-free

    产品特性

    This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    纯化方法

    Purified

    免疫原

    Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

    亚型

    IgG1 kappa
  • 应用备注

    Does not react with chicken or Xenopus, others not known
    • Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    MCF-7, HeLa cells, Breast Cancer

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS (no BSA, no azide)

    储存液

    Azide free
  • 抗原

    Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

    别名

    Cytokeratin 18

    背景

    Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18 Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

    分子量

    45 kDa

    基因ID

    3875, 406013

    UniProt

    P05783

    途径

    Apoptosis, Caspase Cascade in Apoptosis
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