CD55 抗体 (Biotin)
Quick Overview for CD55 抗体 (Biotin) (ABIN6177481)
抗原
See all CD55 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-CD55 (DAF) (143-30), Biotin Conjugate
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产品特性
- This antibody recognizes a single chain glycoprotein of 70 kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- PHA stimulated human PBL
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亚型
- IgG1 kappa
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应用备注
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Immunohistology (frozen) 0.5-1 μg/mL
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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Jurkat, HUT-78, K562, YT, U937, MG63, and human lymphocytes. Human lymph nodes and tonsils.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- CD55 (Complement Decay-Accelerating Factor (CD55))
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别名
- CD55 (DAF)
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分子量
- 70 kDa
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基因ID
- 1604, 126517
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UniProt
- P08174
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途径
- Complement System, Regulation of Leukocyte Mediated Immunity
抗原
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