CD20 抗体 (CF®640R)
Quick Overview for CD20 抗体 (CF®640R) (ABIN6176202)
抗原
See all CD20 (MS4A1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-CD20 (93-1B3), CF640R Conjugate
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产品特性
- This antibody recognizes a protein of 30-33 kDa, which is identified as CD20 (Workshop V, Code CD20.4). It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Stimulated human leukocytes
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亚型
- IgG1 kappa
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应用备注
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Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Functional Studies order Ab without BSA & azide)
- Optimal dilution for a specific application should be determined by user
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说明
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Daudi, Raji, and U266, and human lymphocytes. Lymph nodes and tonsils.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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别名
- CD20
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分子量
- 33-37 kDa
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基因ID
- 931, 712553
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UniProt
- P11836
抗原
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