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CD20 抗体 (CF®405S)

This anti-CD20 antibody is a 小鼠 单克隆 antibody detecting CD20 in FACS, IF 和 Func. Suitable for 人.
产品编号 ABIN6176198
发货至: 中国

Quick Overview for CD20 抗体 (CF®405S) (ABIN6176198)

抗原

See all CD20 (MS4A1) 抗体
CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

适用

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小鼠

克隆类型

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单克隆

标记

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This CD20 antibody is conjugated to CF®405S

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

克隆位点

93-1B3
  • 原理

    Mouse Monoclonal anti-CD20 (93-1B3), CF405S Conjugate

    产品特性

    This antibody recognizes a protein of 30-33 kDa, which is identified as CD20 (Workshop V, Code CD20.4). It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Stimulated human leukocytes

    亚型

    IgG1 kappa
  • 应用备注

    Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Functional Studies order Ab without BSA & azide)
    • Optimal dilution for a specific application should be determined by user

    说明

    Daudi, Raji, and U266, and human lymphocytes. Lymph nodes and tonsils.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

    别名

    CD20

    分子量

    33-37 kDa

    基因ID

    931, 712553

    UniProt

    P11836
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