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kappa Light Chain 抗体 (CF®405S)

适用: 人 FACS, WB, IHC (f) 宿主: 小鼠 Monoclonal HP6053 L1C1 CF®405S
产品编号 ABIN6173918
发货至: 中国
  • 抗原 See all kappa Light Chain 抗体
    kappa Light Chain
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    小鼠
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    单克隆
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    This kappa Light Chain antibody is conjugated to CF®405S
    应用范围
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    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    原理
    Mouse Monoclonal anti-Human Kappa Light Chain (HP6053 + L1C1), CF405S Conjugate
    产品特性
    This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    Purified human Ig kappa chain (HP6053) & Human B-Lymphoma Cells (L1C1)
    克隆位点
    HP6053 L1C1
    亚型
    IgG
    Top Product
    Discover our top product kappa Light Chain Primary Antibody
  • 应用备注
    Immunohistology formalin-fixed 0.5-1.0 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Western blotting 0.5-1.0 μg/mL
    • Flow Cytometry 0.5-2.0 μg/million cells in 0.1 mL
    • Optimal dilution for a specific application should be determined by user
    说明

    293T, Raji or hPBL cells. Tonsil or Spleen

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect from light
  • 抗原
    kappa Light Chain
    Abstract
    kappa Light Chain 产品
    别名
    LOC100037688 antibody, B128L antibody, B55L antibody, IgK antibody, TCRAV31S1 antibody, TCRAV40S1 antibody, kappa light chain antibody, immunoglobulin kappa chain variable 8 (V8) antibody, T cell receptor alpha variable 40 antibody, LOC100037688 antibody, Igk-V8 antibody, TRAV40 antibody
    背景
    HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM
    分子量
    ~22.5 kDa
    基因ID
    3514, 449609
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