Recombinant MLANA 抗体
Quick Overview for Recombinant MLANA 抗体 (ABIN6173499)
抗原
See all MLANA 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
 - Recombinant Rabbit Monoclonal anti-MART-1 / Melan-A / MLANA (MLANA/1409R), Purified, BSA-free
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产品特性
 - This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
 - Purified
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免疫原
 - Recombinant human full-length MLANA protein
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亚型
 - IgG kappa
 
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应用备注
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                        Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min
 - Immunofluorescence 0.5-1 μg/mL
 - Flow Cytometry 0.5-1 μg/million cells/0.1 mL
 - Western blotting 0.5-1 μg/mL
 - Optimal dilution for a specific application should be determined by user
 
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说明
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SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 1 mg/mL
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缓冲液
 - PBS (no BSA, no azide)
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储存液
 - Azide free
 
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- MLANA (Melan A (MLANA))
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别名
 - MART-1 / Melan-A / MLANA
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背景
 - Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
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分子量
 - 20-22 kDa (doublet)
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基因ID
 - 2315, 154069
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UniProt
 - Q16655
 
抗原
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