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MLANA 抗体 (CF®647)

This anti-MLANA antibody is a 小鼠 单克隆 antibody detecting MLANA in WB, IF, FACS, ISt 和 IHC (f). Suitable for 人, 小鼠, 大鼠 和 犬.
产品编号 ABIN6173356
发货至: 中国

Quick Overview for MLANA 抗体 (CF®647) (ABIN6173356)

抗原

See all MLANA 抗体
MLANA (Melan A (MLANA))

适用

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人, 小鼠, 大鼠, 犬

宿主

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小鼠

克隆类型

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单克隆

标记

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This MLANA antibody is conjugated to CF®647

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

MLANA-788
  • 原理

    Mouse Monoclonal anti-MART-1 / Melan-A (MLANA/788), CF647 Conjugate

    产品特性

    This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human MLANA protein

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1.0 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    MLANA (Melan A (MLANA))

    别名

    MART-1 / Melan-A

    背景

    Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA

    分子量

    20-22 kDa (doublet)

    基因ID

    2315, 154069

    UniProt

    Q16655
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