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MLANA 抗体 (Biotin)

MLANA 适用: 人, 小鼠, 大鼠 WB, IF, FACS, ISt, IHC (f) 宿主: 小鼠 Monoclonal M2-7C10 M2-9E3 Biotin
产品编号 ABIN6173263
发货至: 中国
  • 抗原 See all MLANA 抗体
    MLANA (Melan A (MLANA))
    适用
    • 185
    • 102
    • 84
    • 33
    • 15
    人, 小鼠, 大鼠
    宿主
    • 97
    • 87
    • 1
    小鼠
    克隆类型
    • 114
    • 71
    单克隆
    标记
    • 76
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    This MLANA antibody is conjugated to Biotin
    应用范围
    • 133
    • 101
    • 100
    • 77
    • 59
    • 52
    • 28
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    • 26
    • 22
    • 13
    • 11
    • 9
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    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    原理
    Mouse Monoclonal anti-MART-1 / Melan-A (M2-7C10 + M2-9E3), Biotin Conjugate
    产品特性
    This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    Recombinant hMART-1 protein (M2-7C10, M2-9E3)
    克隆位点
    M2-7C10 M2-9E3
    亚型
    IgG
    Top Product
    Discover our top product MLANA Primary Antibody
  • 应用备注
    Immunohistology formalin-fixed 0.5-1 μg/mL /mL
    • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1.0 μg/mL
    • Optimal dilution for a specific application should be determined by user
    说明

    SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原
    MLANA (Melan A (MLANA))
    别名
    MART-1 / Melan-A (MLANA 产品)
    别名
    A930034P04Rik antibody, Mart1 antibody, MART-1 antibody, MART1 antibody, melan-A antibody, Mlana antibody, MLANA antibody
    背景
    Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
    分子量
    20-22 kDa (doublet)
    基因ID
    2315, 154069
    UniProt
    Q16655
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