MLANA 抗体 (CF®647)
Quick Overview for MLANA 抗体 (CF®647) (ABIN6173242)
抗原
See all MLANA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
- 
    
- 
                                            
原理
 - Mouse Monoclonal anti-MART-1 / Melan-A (M2-7C10 + M2-9E3), CF647 Conjugate
 - 
                                            
产品特性
 - This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
 - 
                                            
免疫原
 - Recombinant hMART-1 protein (M2-7C10, M2-9E3)
 - 
                                            
亚型
 - IgG
 
 - 
                                            
 - 
    
 - 
    
- 
                                            
应用备注
 - 
                        Immunohistology formalin-fixed 0.5-1 μg/mL /mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
 - Immunofluorescence 0.5-1 μg/mL
 - Flow Cytometry 0.5-1 μg/million cells/0.1 mL
 - Western blotting 0.5-1.0 μg/mL
 - Optimal dilution for a specific application should be determined by user
 
 - 
                                            
说明
 - 
                        
SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas
 - 
                                            
限制
 - 仅限研究用
 
 - 
                                            
 - 
    
- 
                                            
状态
 - Liquid
 - 
                                            
浓度
 - 100 μg/mL
 - 
                                            
缓冲液
 - PBS/0.1 % BSA/0.05 % azide
 - 
                                            
储存液
 - Sodium azide
 - 
                                            
注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
 - 
                                            
注意事项
 - Protect from light
 
 - 
                                            
 - 
    
- MLANA (Melan A (MLANA))
 - 
                                            
别名
 - MART-1 / Melan-A
 - 
                                            
背景
 - Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
 - 
                                            
分子量
 - 20-22 kDa (doublet)
 - 
                                            
基因ID
 - 2315, 154069
 - 
                                            
UniProt
 - Q16655
 
抗原
 -