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NEFL 抗体 (Biotin)

This anti-NEFL antibody is a 小鼠 单克隆 antibody detecting NEFL in IF, FACS 和 IHC (f). Suitable for 人, 大鼠, Pig, Cow 和 小鸡.
产品编号 ABIN6172845
发货至: 中国

Quick Overview for NEFL 抗体 (Biotin) (ABIN6172845)

抗原

See all NEFL 抗体
NEFL (Neurofilament, Light Polypeptide (NEFL))

适用

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人, 大鼠, Pig, Cow, 小鸡

宿主

  • 90
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小鼠

克隆类型

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单克隆

标记

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This NEFL antibody is conjugated to Biotin

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

NFL-736
  • 原理

    Mouse Monoclonal anti-Neurofilament (NF-L) (NFL/736), Biotin Conjugate

    产品特性

    This MAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human NEFL protein

    亚型

    IgG1
  • 应用备注

    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 1-2 μg/mL,Flow Cytometry
    • Optimal dilution for a specific application should be determined by user

    说明

    Brain, Neuroblastoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    NEFL (Neurofilament, Light Polypeptide (NEFL))

    别名

    Neurofilament (NF-L)

    分子量

    68 kDa

    基因ID

    4747, 521461

    UniProt

    P07196
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