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KRT14 抗体 (C-Term) (CF®568)

This anti-KRT14 antibody is a 小鼠 单克隆 antibody detecting KRT14 in IF, FACS 和 IHC (f). Suitable for 人, 大鼠 和 小鼠.
产品编号 ABIN6172564
发货至: 中国

Quick Overview for KRT14 抗体 (C-Term) (CF®568) (ABIN6172564)

抗原

See all KRT14 抗体
KRT14 (Keratin 14 (KRT14))

适用

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人, 大鼠, 小鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This KRT14 antibody is conjugated to CF®568

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

LL002
  • 抗原表位

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    C-Term

    原理

    Mouse Monoclonal anti-Cytokeratin 14 (LL002), CF568 Conjugate

    产品特性

    Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.

    亚型

    IgG3
  • 应用备注

    Immunohistology formalin-fixed 0.5-1.0 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    A431 cells. Skin or Squamous cell carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    KRT14 (Keratin 14 (KRT14))

    别名

    Cytokeratin 14

    背景

    CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, k14, Keratin 14, Keratin Type I Cytoskeletal 14, Koebner, krt14, NFJ

    分子量

    ~50 kDa

    基因ID

    3861, 6545380

    UniProt

    P02533
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