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KRT15 抗体 (C-Term) (CF®647)

This anti-KRT15 antibody is a 小鼠 单克隆 antibody detecting KRT15 in WB, FACS, IF 和 IHC (fro). Suitable for 人, 大鼠 和 Cow.
产品编号 ABIN6172482
发货至: 中国

Quick Overview for KRT15 抗体 (C-Term) (CF®647) (ABIN6172482)

抗原

See all KRT15 抗体
KRT15 (Keratin 15 (KRT15))

适用

  • 154
  • 47
  • 27
  • 27
  • 2
人, 大鼠, Cow

宿主

  • 88
  • 71
  • 2
小鼠

克隆类型

  • 95
  • 66
单克隆

标记

  • 64
  • 19
  • 7
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This KRT15 antibody is conjugated to CF®647

应用范围

  • 91
  • 91
  • 62
  • 52
  • 42
  • 19
  • 16
  • 15
  • 14
  • 14
  • 13
  • 10
  • 6
  • 5
  • 2
  • 1
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

LHK15
  • 抗原表位

    • 18
    • 15
    • 7
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    原理

    Mouse Monoclonal anti-Cytokeratin 15 (LHK15), CF647 Conjugate

    产品特性

    Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    A 17 amino acids synthetic peptide from the C- terminal of Human Cytokeratin 15 (exact sequence is proprietary)

    亚型

    IgG2a kappa
  • 应用备注

    Immunohistology (frozen or formalin) 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    A431 cells. Skin or Colon

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    KRT15 (Keratin 15 (KRT15))

    别名

    Cytokeratin 15

    背景

    CK15, Cytokeratin 15, K1CO, Ka15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15

    分子量

    52 kDa

    基因ID

    3866, 654570

    UniProt

    P19012
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