Keratin 10 抗体 (CF®594)
Quick Overview for Keratin 10 抗体 (CF®594) (ABIN6172384)
抗原
See all Keratin 10 (KRT10) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Cytokeratin 10 (KRT10/844), CF594 Conjugate
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产品特性
- This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human KRT10 protein
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亚型
- IgG1 kappa
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anti-Keratin 10 (KRT10) (AA 153-456) antibody
KRT10 适用: 人 IHC, WB, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼 IHC, WB, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal RKSE60 unconjugated
anti-Keratin 10 (KRT10) (AA 345-454) antibodyKRT10 适用: 人, 小鼠, 大鼠, 猴 IHC, WB, FACS, ELISA 宿主: 小鼠 Monoclonal 3C2F5 unconjugated
anti-Keratin 10 (KRT10) (AA 1-584) antibodyKRT10 适用: 人 IHC, WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠, 大鼠, 犬, Pig IHC, WB, FACS, ICC, IHC (fro) 宿主: 小鼠 Monoclonal RKSE60 unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 1D8 unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人, 小鼠 IHC, WB, StM 宿主: 小鼠 Monoclonal LH2 unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人 IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VIK-10 unconjugated
anti-Keratin 10 (KRT10) (Internal Region) antibodyKRT10 适用: 人, 小鼠, 大鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 10 (KRT10) antibodyKRT10 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-610M unconjugated Recombinant Antibody
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应用备注
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Immunohistology formalin-fixed 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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A431, HeLa, MCF7 cells. Esophagus or tonsil.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Keratin 10 (KRT10)
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别名
- Cytokeratin 10
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背景
- BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10
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分子量
- 56.5 kDa
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基因ID
- 3858, 99936
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UniProt
- P13645
抗原
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