Keratin 6 抗体 (C-Term) (CF®594)
Quick Overview for Keratin 6 抗体 (C-Term) (CF®594) (ABIN6170978)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- C-Term
-
原理
- Mouse Monoclonal anti-Cytokeratin 6 (LHK6), CF594 Conjugate
-
产品特性
- This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6
-
亚型
- IgG2a kappa
-
-
anti-Keratin 6 (KRT6) antibody
KRT6 适用: 人, 小鼠, 大鼠 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Keratin 6 (KRT6) antibodyKRT6 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-606R unconjugated Recombinant Antibody
anti-Keratin 6 (KRT6) antibodyKRT6 适用: 人 WB 宿主: 小鼠 Monoclonal 4A1 unconjugated
anti-Keratin 6 (KRT6) (C-Term) antibodyKRT6 适用: 人, 小鼠 IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal LHK6 unconjugated
-
-
应用备注
-
Immunohistology formalin-fixed 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
-
说明
-
Tonsil or basal cell carcinoma
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 100 μg/mL
-
缓冲液
- PBS/0.1 % BSA/0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
-
- Keratin 6 (KRT6)
-
别名
- Cytokeratin 6
-
分子量
- 56 kDa
-
基因ID
- 700779
抗原
-
