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Cytokeratin 7 抗体 (CF®594)

This CF®594-conjugated 小鼠 单克隆 anti-Cytokeratin 7 antibody (Clone K72-7) specifically detects Cytokeratin 7 in IHC, IF 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN6170028
发货至: 中国
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Quick Overview for Cytokeratin 7 抗体 (CF®594) (ABIN6170028)

抗原

See all Cytokeratin 7 (KRT7) 抗体
Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 7 antibody is conjugated to CF®594

应用范围

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Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

K72-7
  • 原理

    Mouse Monoclonal anti-Cytokeratin 7 (K72.7), CF594 Conjugate

    产品特性

    This antibody recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7 ) from colon carcinomas (keratin 7-). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Semi-purified cytokeratin preparation.

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology (formalin)
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    HeLa cells. Carcinoma of ovary, lung, cervix, or breast

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

    别名

    Cytokeratin 7

    背景

    CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

    分子量

    55 kDa

    基因ID

    3855, 411501

    UniProt

    P08729
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