Podoplanin 抗体 (AA 24-126) (CF®594)
Quick Overview for Podoplanin 抗体 (AA 24-126) (CF®594) (ABIN6167005)
抗原
See all Podoplanin (PDPN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 24-126
-
原理
- Mouse Monoclonal anti-Podoplanin (PDPN/1433), CF594 Conjugate
-
产品特性
- This antibody recognizes a muco-protein of 38-43 kDa, which is identified Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- Recombinant human Podoplanin (PDPN) protein fragment (aa24-126) (exact sequence is proprietary)
-
亚型
- IgG1 kappa
-
-
-
-
应用备注
-
Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
-
说明
-
HeLa Cells. Angiosarcoma or Mesothelioma.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 100 μg/mL
-
缓冲液
- PBS/0.1 % BSA/0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
-
- Podoplanin (PDPN)
-
别名
- Podoplanin
-
分子量
- 38-43 kDa
-
基因ID
- 10630, 468675
-
UniProt
- Q86YL7
-
途径
- Dicarboxylic Acid Transport
抗原
-