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Podoplanin 抗体 (AA 24-126) (CF®568)

This CF®568-conjugated 小鼠 单克隆 anti-Podoplanin antibody (Clone PDPN-1433) specifically detects Podoplanin in WB, IHC, FACS 和 IF. The antibody is reactive with 人 samples.
产品编号 ABIN6166995
发货至: 中国
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Quick Overview for Podoplanin 抗体 (AA 24-126) (CF®568) (ABIN6166995)

抗原

See all Podoplanin (PDPN) 抗体
Podoplanin (PDPN)

适用

  • 99
  • 30
  • 8
  • 3
  • 1
  • 1

宿主

  • 72
  • 33
  • 6
  • 6
  • 2
  • 2
  • 2
小鼠

克隆类型

  • 72
  • 50
  • 1
单克隆

标记

  • 74
  • 12
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  • 3
  • 3
  • 3
  • 3
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  • 1
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This Podoplanin antibody is conjugated to CF®568

应用范围

  • 94
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Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

克隆位点

PDPN-1433
  • 抗原表位

    • 12
    • 9
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    • 3
    • 2
    • 2
    • 2
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    • 1
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    • 1
    AA 24-126

    原理

    Mouse Monoclonal anti-Podoplanin (PDPN/1433), CF568 Conjugate

    产品特性

    This antibody recognizes a muco-protein of 38-43 kDa, which is identified Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human Podoplanin (PDPN) protein fragment (aa24-126) (exact sequence is proprietary)

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology (formalin) 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Immunofluorescence 0.5-1 μg/mL Western blotting 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    HeLa Cells. Angiosarcoma or Mesothelioma.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    Podoplanin (PDPN)

    别名

    Podoplanin

    分子量

    38-43 kDa

    基因ID

    10630, 468675

    UniProt

    Q86YL7

    途径

    Dicarboxylic Acid Transport
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