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Vimentin 抗体

The 小鼠 单克隆 anti-Vimentin antibody is suitable to detect Vimentin in samples from 人, 大鼠, 小鼠, Pig, Cow, Cat, 兔 和 绵羊. It has been validated for IF, FACS 和 IHC (f).
产品编号 ABIN6166864
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Vimentin 抗体 (ABIN6166864)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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人, 大鼠, 小鼠, Pig, Cow, Cat, 兔, 绵羊

宿主

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小鼠

克隆类型

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单克隆

标记

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This Vimentin antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

LN-6
  • 原理

    Mouse Monoclonal anti-Vimentin (LN-6), Purified, with BSA

    产品特性

    This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    纯化方法

    Purified

    免疫原

    Human thymic nuclear extract

    亚型

    IgM
  • 应用备注

    Immunohistology formalin-fixed 1-2 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Jurkat cells, Sarcomas, Melanomas

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    200 μg/mL

    缓冲液

    PBS/0.05 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    Vimentin (VIM)

    别名

    Vimentin

    背景

    VIM

    分子量

    57-60 kDa

    基因ID

    7431, 455493

    UniProt

    P08670

    途径

    Caspase Cascade in Apoptosis
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