Vimentin 抗体
-
- 抗原 See all Vimentin (VIM) 抗体
- Vimentin (VIM)
-
适用
- 人, 小鼠, 大鼠, Pig, Cow, Cat, 兔, 绵羊
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Vimentin antibody is un-conjugated
-
应用范围
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-Vimentin (LN-6), Purified, with BSA
- 产品特性
- This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Human thymic nuclear extract
- 克隆位点
- LN-6
- 亚型
- IgM
- Top Product
- Discover our top product VIM Primary Antibody
-
anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyKO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) (AA 435-466), (C-Term) antibodyVIM 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C8 unconjugated
anti-Vimentin (VIM) (AA 41-140) antibodyVIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated
anti-Vimentin (VIM) antibodyVIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody
anti-Vimentin (VIM) antibodyKO Validated VIM 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Vimentin (VIM) (AA 210-466) antibodyVIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated
anti-Vimentin (VIM) (AA 2-466) antibodyVIM 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3A1F2 unconjugated
-
- 应用备注
-
Immunohistology formalin-fixed 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
-
Jurkat cells, Sarcomas, Melanomas
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- PBS/0.05 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
- 抗原
- Vimentin (VIM)
- 别名
- Vimentin (VIM 产品)
- 背景
- VIM
- 分子量
- 57-60 kDa
- 基因ID
- 7431, 455493
- UniProt
- P08670
- 途径
- Caspase Cascade in Apoptosis
-
