TIMP2 抗体 (N-Term)
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- 抗原 See all TIMP2 抗体
- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
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抗原表位
- AA 56-66, N-Term
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适用
- 人, 大鼠, 小鼠, Cow, 斑马鱼, 兔, 豚鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TIMP2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Mouse Monoclonal anti-TIMP2 (3A4), Purified, BSA-free
- 产品特性
- This antibody recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)
- 克隆位点
- 3A4
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product TIMP2 Primary Antibody
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- 应用备注
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Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (no BSA, no azide)
- 储存液
- Azide free
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- 抗原
- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
- 别名
- TIMP2 (TIMP2 产品)
- 背景
- Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, TIMP2, Tissue Inhibitor of Metalloproteinase 2
- 分子量
- 21 kDa
- 基因ID
- 7077, 633514
- UniProt
- P16035
- 途径
- cAMP Metabolic Process
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