Involucrin 抗体
Quick Overview for Involucrin 抗体 (ABIN6164850)
抗原
See all Involucrin (IVL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Mouse Monoclonal anti-Involucrin (SY5), Purified, with BSA
-
产品特性
- This antibody recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
纯化方法
- Purified
-
免疫原
- Purified involucrin from human keratinocytes
-
亚型
- IgG1 kappa
-
-
anti-Involucrin (IVL) (AA 461-551) antibody
IVL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Involucrin (IVL) (AA 65-180) antibodyIVL 适用: 人 ELISA, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 301-460) antibodyIVL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 24-118) antibodyIVL 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) (AA 36-152) antibodyIVL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Involucrin (IVL) antibodyIVL 适用: 人, 犬, Pig, Gorilla, Owl Monkey IHC, ISt, StM 宿主: 小鼠 Monoclonal rIVRN-827 unconjugated Recombinant Antibody
anti-Involucrin (IVL) antibodyIVL 适用: 人, 犬, Pig, 猴 WB, IHC, ELISA, IP, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal SY5 unconjugated
-
-
应用备注
-
Immunohistology formalin-fixed 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1 mg/mL PBS for 5 min at 37C, enzyme digestion is better than citrate buffer based epitope unmasking
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence
- Does not react with mouse, others not known
- Optimal dilution for a specific application should be determined by user
-
说明
-
MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 200 μg/mL
-
缓冲液
- PBS/0.05 % BSA/0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
-
- Involucrin (IVL)
-
别名
- Involucrin
-
背景
- IVL
-
分子量
- 66-170 kDa
-
基因ID
- 3713, 516439
-
UniProt
- P07476
抗原
-
