Mitochondrial Marker 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in IF, IHC (f), WB, FACS 和 ISt. Suitable for 人, 小鼠 和 大鼠.
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Quick Overview for Mitochondrial Marker 抗体 (ABIN6164016)
抗原
Mitochondrial Marker
适用
人, 小鼠, 大鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB), Flow Cytometry (FACS), Immunostaining (ISt)
克隆位点
MTC719
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原理
- Mouse Monoclonal anti-Mitochondrial Marker (MTC719), Purified, BSA-free
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产品特性
- This MAb recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
- Purified
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免疫原
- Mitochondrial fraction of HeLa cells
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亚型
- IgG1 kappa
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anti-Mitochondrial Marker antibody
适用: 人 IF, WB, FACS, ISt, IHC, StM 宿主: 小鼠 Monoclonal MTC719 unconjugated
anti-Mitochondrial Marker antibody适用: 人 IF, IHC (f), WB, Coat, ELISA 宿主: 小鼠 Monoclonal AE-1 unconjugated
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应用备注
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Does not react with insects or bacteria
- Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.25-0.5 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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HeLa or HepG2 cells. Hepatic carcinomas.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS (no BSA, no azide)
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储存液
- Azide free
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- Mitochondrial Marker
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分子量
- 60 kDa
抗原
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