Mitochondrial Marker 抗体 (Biotin)
Quick Overview for Mitochondrial Marker 抗体 (Biotin) (ABIN6163932)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Mitochondrial Marker (AE-1), Biotin Conjugate
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产品特性
- This MAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Semi-purified mitochondrial preparation
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亚型
- IgG1 kappa
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anti-Mitochondrial Marker antibody
适用: 人 IF, WB, FACS, ISt, IHC, StM 宿主: 小鼠 Monoclonal MTC719 unconjugated
anti-Mitochondrial Marker antibody适用: 人, 小鼠, 大鼠 IF, IHC (f), WB, FACS, ISt 宿主: 小鼠 Monoclonal MTC719 unconjugated
anti-Mitochondrial Marker antibody适用: 人 IF, IHC (f), WB, Coat, ELISA 宿主: 小鼠 Monoclonal AE-1 unconjugated
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应用备注
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Does not react with mouse or rat, others not known
- Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- ELISA For coating, order Ab without BSA
- Western blotting 0.25-0.5 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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HeLa or HepG2 cells. Hepatic carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Mitochondrial Marker
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分子量
- 60 kDa
抗原
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