VWF 抗体 (AA 845-949) (Biotin)
-
- 抗原 See all VWF 抗体
- VWF (Von Willebrand Factor (VWF))
-
抗原表位
- AA 845-949
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This VWF antibody is conjugated to Biotin
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)
- 原理
- Mouse Monoclonal anti-von Willebrand Factor (3E2D10), Biotin Conjugate
- 产品特性
- von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Recombinant human vWF fragment spanning aa 845-949
- 克隆位点
- 3E2D10
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product VWF Primary Antibody
-
-
- 应用备注
-
Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1.0 μg/mL
- Immunoprecipitation 0.5-1 μg/500 μg protein lysate
- Optimal dilution for a specific application should be determined by user
- 说明
-
HUVEC cells or Tonsil
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
- 抗原
- VWF (Von Willebrand Factor (VWF))
- 别名
- von Willebrand Factor (VWF 产品)
- 别名
- VWF antibody, si:ch1073-474e24.1 antibody, F8VWF antibody, VWD antibody, 6820430P06Rik antibody, AI551257 antibody, B130011O06Rik antibody, C630030D09 antibody, von Willebrand factor antibody, Von Willebrand factor antibody, VWF antibody, vwf antibody, Vwf antibody
- 背景
- Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)
- 分子量
- 250 kDa
- 基因ID
- 7450, 440848
- UniProt
- P04275
-