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CD6 抗体 (CF®405S)

This CF®405S-conjugated 小鼠 单克隆 anti-CD6 antibody (Clone 3F7B5) specifically detects CD6 in FACS, IF 和 IHC (f). The antibody is reactive with 人 samples.
产品编号 ABIN6160065
发货至: 中国
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Quick Overview for CD6 抗体 (CF®405S) (ABIN6160065)

抗原

See all CD6 抗体
CD6

适用

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宿主

  • 69
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小鼠

克隆类型

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单克隆

标记

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This CD6 antibody is conjugated to CF®405S

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

3F7B5
  • 原理

    Mouse Monoclonal anti-CD6 (3F7B5), CF405S Conjugate

    产品特性

    CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular ?scavenger receptor cysteine-rich? (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Human rheumatoid synovial T cell line ST-1

    亚型

    IgG1
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    CCRF-CEM, Jurkat cells, Tonsil

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    CD6

    别名

    CD6

    背景

    T12, TP120

    分子量

    90-130 kDa

    基因ID

    923, 744366

    UniProt

    P30203
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