UBE2F 抗体 (N-Term)
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Quick Overview for UBE2F 抗体 (N-Term) (ABIN615974)
抗原
See all UBE2F 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- SKLKRDDGLK GSRT-C
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特异性
- Recogfnizes NEDD8 conjugating enzyme (N Terminus).
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交叉反应 （详细）
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Species reactivity (expected):Human, Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Rat. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence from the N Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F
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anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) antibody
UBE2F 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) antibodyKD Validated UBE2F 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB940 unconjugated Recombinant Antibody
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) antibodyUBE2F 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) antibodyUBE2F 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyVerified UBE2F 适用: 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (C-Term) antibodyVerified UBE2F 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) antibodyUBE2F 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 猴, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (C-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, 猴, Bat, Hamster, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
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别名
- NCE2
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背景
- Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F
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基因ID
- 140739
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NCBI登录号
- NP_542409
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UniProt
- Q969M7
抗原
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