PLAP 抗体 (CF®594)
Quick Overview for PLAP 抗体 (CF®594) (ABIN6155947)
抗原
See all PLAP (ALPP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Mouse Monoclonal anti-PLAP (Placental Alkaline Phosphatase) (ALPP/238), CF594 Conjugate
-
产品特性
- PLAP is a tissue specific, trophoblast-derived, 70 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2 metallo glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98 % homologous. Non-tissue specific AP is found in kidney, liver and bone. This MAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP as to AP from normal human testis. This MAb can be used both as coating as well as tracer antibody in the same ELISA to detect PLAP in serum of S, F, FS and I phenotypes. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- Recombinant full-length human ALPP protein
-
亚型
- IgG2a kappa
-
-
-
-
应用备注
-
Immunohistology frozen 1-2 μg/mL for 30 minutes at RT
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
-
说明
-
HepG2 cells. Placenta or seminoma
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 100 μg/mL
-
缓冲液
- PBS/0.1 % BSA/0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
-
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
-
别名
- PLAP (Placental Alkaline Phosphatase)
-
分子量
- 70 kDa
-
基因ID
- 250, 284255
-
UniProt
- P05187
抗原
-