ENO2/NSE 抗体 (AA 416-433) (CF®640R)
Quick Overview for ENO2/NSE 抗体 (AA 416-433) (CF®640R) (ABIN6153609)
抗原
See all ENO2/NSE (ENO2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 416-433
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原理
- Mouse Monoclonal anti-NSE-gamma (ENO2/1375), CF640R Conjugate
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产品特性
- This antibody recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- A synthetic peptide corresponding to aa416-433 of human NSE gamma (exact sequence is proprietary)
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亚型
- IgG2b
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anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibody
ENO2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-434) antibodyENO2 适用: 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 325-434) antibodyENO2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IHC, StM 宿主: 小鼠 Monoclonal ENO2-1375 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 251-433) antibodyENO2 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5D3 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) antibodyENO2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 2-285) antibodyENO2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 416-433) antibodyENO2 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal ENO2-1375 unconjugated
anti-Enolase 2 (Gamma, Neuronal) (ENO2) (AA 1-434) antibodyENO2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistology (formalin) 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- NSE-gamma
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背景
- 2-phospho-D-glycerate hydrolyase, ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE
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分子量
- ~50 kDa
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基因ID
- 2026, 511915
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UniProt
- P09104
抗原
-
