HLA-Aw32-&-HLA-A25 (MHC-I) 抗体 (CF®640R)
This anti- antibody is a 小鼠 单克隆 antibody detecting in FACS, IF 和 IHC (f). Suitable for 人.
Quick Overview for HLA-Aw32-&-HLA-A25 (MHC-I) 抗体 (CF®640R) (ABIN6152735)
抗原
HLA-Aw32-&-HLA-A25 (MHC-I)
适用
人
宿主
小鼠
克隆类型
单克隆
标记
CF®640R
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
克隆位点
CATA-1
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原理
- Mouse Monoclonal anti-HLA-Aw32-&-HLA-A25 (MHC-I) (CATA-1), CF640R Conjugate
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产品特性
- This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10
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亚型
- IgG2a kappa
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anti-HLA-Aw32-&-HLA-A25 (MHC-I) antibody
适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal CATA-1 unconjugated
anti-HLA-Aw32-&-HLA-A25 (MHC-I) antibody适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal CATA-1 unconjugated
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应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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THP-1 and Jurkat cells. Tonsil or lymph node.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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