HLA-Aw32-&-HLA-A25 (MHC-I) 抗体
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Quick Overview for HLA-Aw32-&-HLA-A25 (MHC-I) 抗体 (ABIN3026977)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- HLA-A25 Antibody (HLA-Aw32)
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产品特性
- This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, and DRw10 were used as the immunogen for the HLA-A25 antibody (HLA-Aw32).
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亚型
- IgG2a, kappa
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anti-HLA-Aw32-&-HLA-A25 (MHC-I) antibody
Carrier-free 适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal CATA-1 unconjugated
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应用备注
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Optimal dilution of the HLA-A25 antibody (HLA-Aw32) should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the HLA-A25 antibody (HLA-Aw32) at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- HLA-Aw32-&-HLA-A25 (MHC-I)
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别名
- HLA-A25 (HLA-Aw32)
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背景
- This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.
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UniProt
- P30443
抗原
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