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LGALS1/Galectin 1 抗体 (AA 12-108)

This anti-LGALS1/Galectin 1 antibody is a 小鼠 单克隆 antibody detecting LGALS1/Galectin 1 in WB, IHC, ELISA, FACS, IF 和 Coat. Suitable for 人.
产品编号 ABIN6152690
发货至: 中国
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Quick Overview for LGALS1/Galectin 1 抗体 (AA 12-108) (ABIN6152690)

抗原

See all LGALS1/Galectin 1 (LGALS1) 抗体
LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This LGALS1/Galectin 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat)

克隆位点

GAL1-1831
  • 抗原表位

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    AA 12-108

    原理

    Mouse Monoclonal anti-Galectin-1 / Human Placental Lactogen (GAL1/1831), Purified, with BSA

    产品特性

    Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 Molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    纯化方法

    Purified

    免疫原

    Recombinant human Galectin-1 protein fragment (aa12-108) (exact sequence is proprietary)

    亚型

    IgG Mix lambda
  • 应用备注

    Flow cytometry 0.5-1 μg/million cells in 0.1 mL
    • Immunohistology (formalin) 0.5-1 μg/mL
    • ELISA 2-4 μg/mL for coating (order Ab without BSA)
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    200 μg/mL

    缓冲液

    PBS/0.05 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

    别名

    Galectin-1 / Placental Lactogen

    分子量

    14 kDa

    基因ID

    3956, 445351

    UniProt

    P09382

    途径

    Carbohydrate Homeostasis
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