LGALS1/Galectin 1 抗体 (AA 12-108) (CF®405S)
Quick Overview for LGALS1/Galectin 1 抗体 (AA 12-108) (CF®405S) (ABIN6152655)
抗原
See all LGALS1/Galectin 1 (LGALS1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 12-108
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原理
- Mouse Monoclonal anti-Galectin-1 / Human Placental Lactogen (GAL1/1831), CF405S Conjugate
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产品特性
- Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 Molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human Galectin-1 protein fragment (aa12-108) (exact sequence is proprietary)
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亚型
- IgG Mix lambda
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应用备注
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Flow cytometry 0.5-1 μg/million cells in 0.1 mL
- Immunohistology (formalin) 0.5-1 μg/mL
- ELISA 2-4 μg/mL for coating (order Ab without BSA)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
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别名
- Galectin-1 / Placental Lactogen
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分子量
- 14 kDa
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基因ID
- 3956, 445351
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UniProt
- P09382
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途径
- Carbohydrate Homeostasis
抗原
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