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CD166 抗体 (AA 28-527) (Biotin)

The Biotin-conjugated 小鼠 单克隆 anti-CD166 antibody (ABIN602949) specifically detects CD166 in WB 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN602949
发货至: 中国
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北京 101111
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Room 801-803
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Quick Overview for CD166 抗体 (AA 28-527) (Biotin) (ABIN602949)

抗原

See all CD166 (ALCAM) 抗体
CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD166 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Flow Cytometry (FACS)
  • 抗原表位

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    AA 28-527

    特异性

    Detects Recombinant, Human ALCAM in Western blots. No cross-reactivity seen with Recombinant, Murine ALCAM, rhBCAM, rhTROP-2, rhEpCAM, rhMCAM, rhNCAML1, r  mMadCAM or rmOCAM.

    纯化方法

    Immunoaffinity purified

    免疫原

    Purified, NS0-derived, recombinant human ALCAM extracellular domain (rhALCAM, aa 28-527, Accession No. Q13740).

    亚型

    IgG1
  • 应用备注

    Approved: Flo (25 μg/mL), WB (1 - 2 μg/mL)

    Usage: Suitable for use in Western Blot, Flow Cytometry. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhALCAM is approximately 5 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow cytometry: 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile PBS

    浓度

    Lot specific

    缓冲液

    Sterile-filtered, lyophilized powder in PBS,  pH 7.2, BSA

    储存条件

    4 °C,-20 °C

    储存方法

    Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
  • 抗原

    CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

    别名

    ALCAM / CD166

    背景

    Name/Gene ID: ALCAM

    Synonyms: ALCAM, CD166 antigen, MEMD, CD166

    基因ID

    214

    UniProt

    Q13740
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