CD166 抗体 (AA 28-527) (Biotin)
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- 抗原 See all CD166 (ALCAM) 抗体
- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
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抗原表位
- AA 28-527
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD166 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS)
- 特异性
- Detects Recombinant, Human ALCAM in Western blots. No cross-reactivity seen with Recombinant, Murine ALCAM, rhBCAM, rhTROP-2, rhEpCAM, rhMCAM, rhNCAML1, r mMadCAM or rmOCAM.
- 纯化方法
- Immunoaffinity purified
- 免疫原
- Purified, NS0-derived, recombinant human ALCAM extracellular domain (rhALCAM, aa 28-527, Accession No. Q13740).
- 亚型
- IgG1
- Top Product
- Discover our top product ALCAM Primary Antibody
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- 应用备注
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Approved: Flo (25 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhALCAM is approximately 5 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow cytometry: 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile PBS
- 浓度
- Lot specific
- 缓冲液
- Sterile-filtered, lyophilized powder in PBS, pH 7.2, BSA
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
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