CD166 抗体 (AA 28-527) (Biotin)
Quick Overview for CD166 抗体 (AA 28-527) (Biotin) (ABIN602949)
抗原
See all CD166 (ALCAM) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 28-527
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特异性
- Detects Recombinant, Human ALCAM in Western blots. No cross-reactivity seen with Recombinant, Murine ALCAM, rhBCAM, rhTROP-2, rhEpCAM, rhMCAM, rhNCAML1, r mMadCAM or rmOCAM.
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纯化方法
- Immunoaffinity purified
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免疫原
- Purified, NS0-derived, recombinant human ALCAM extracellular domain (rhALCAM, aa 28-527, Accession No. Q13740).
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亚型
- IgG1
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应用备注
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Approved: Flo (25 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhALCAM is approximately 5 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow cytometry: 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS
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浓度
- Lot specific
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缓冲液
- Sterile-filtered, lyophilized powder in PBS, pH 7.2, BSA
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储存条件
- 4 °C,-20 °C
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储存方法
- Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
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- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
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别名
- ALCAM / CD166
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背景
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Name/Gene ID: ALCAM
Synonyms: ALCAM, CD166 antigen, MEMD, CD166 -
基因ID
- 214
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UniProt
- Q13740
抗原
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