CSPG5 抗体 (AA 31-420)
Quick Overview for CSPG5 抗体 (AA 31-420) (ABIN601028)
抗原
See all CSPG5 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 31-420
-
特异性
- Recognizes Neuroglycan C, extracellular domain. In direct ELISA, this antibody shows 25 % cross-reactivity with rhNeuroglycan C. Species cross-reactivity: Mouse, rat.
-
纯化方法
- Affinity purified
-
过滤
- Sterile filtered
-
免疫原
- Purified, CHO cell-derived, recombinant mouse Neuroglycan C (rmNeuroglycan C, aa 31-420, Accession# AAH55736).
-
亚型
- IgG
-
-
-
-
应用备注
-
Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (1 μg/mL)
Usage: Suitable for Immunohistochemistry ELISA and Western Blot. ELISA: 0.5-1 μg/mL. Western Blot: 1 μg/mL. Immunohistochemistry: 10 μg/mL detects Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked. with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-goat lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA. -
说明
-
Target Species of Antibody: Mouse
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Sterile PBS
-
浓度
- Lot specific
-
缓冲液
- Lyophilized from a 0.2 um sterile-filtered solution in PBS, 5 % trehalose
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
-
-
- CSPG5 (Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5))
-
别名
- CSPG5 / Neuroglycan C
-
背景
-
Name/Gene ID: CSPG5
Synonyms: CSPG5, CALEB, Neuroglycan C, NGC -
基因ID
- 10675
-
UniProt
- O95196
-
途径
- Glycosaminoglycan Metabolic Process
抗原
-