CSPG5 抗体 (Extracellular Domain)
Quick Overview for CSPG5 抗体 (Extracellular Domain) (ABIN601027)
抗原
See all CSPG5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Extracellular Domain
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特异性
- Recognizes human Neuroglycan C. Shows approximately 50 % cross-reactivity with recombinant mouse Neuroglycan C in direct ELISA.
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纯化方法
- Immunoaffinity purified
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过滤
- Sterile filtered
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免疫原
- CHO cell-derived rhNeuroglycan C extracellular domain.
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亚型
- IgG
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anti-Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5) (AA 139-370) antibody
CSPG5 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5) (AA 351-450) antibodyCSPG5 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5) (AA 445-539) antibodyCSPG5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G6 unconjugated
anti-Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5) (AA 445-539) antibodyCSPG5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5) (AA 108-157) antibodyCSPG5 适用: 人, 大鼠, 小鼠, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)
Usage: Applications:Suitable for use in ELISA, Immunocytochemistry and WesternBlot. Other Applications not tested.Recommended Dilution:Direct ELISA: 0.5-1 μg/mL. The detection limit for recombinanthuman Neuroglycan C is approximately 0.3 ng/well.Western Blot: 1 μg/mLImmunocytochemistry: 10 μg/mL was used to detect NeuroglycanC in sections of adult mouse cerebellum. Cells were fixed withPBS containing 4 % paraformaldehyde for 20 minutes at roomtemperature and blocked with PBS containing 10 % normaldonkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutesat RT, After blocking, cells were incubated with dilutedLS-C123249 for 3 hours at RT followed by NorthernLights 557donkey anti-sheep lgG at RT for 1 hour. Between each step, cellswere washed with PBS containing 0.1 % BSA.Optimal dilutions to be determined by the researcher -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile 40-50 % glycerol.
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浓度
- Lot specific
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缓冲液
- Lyophilized from a 0.2 um sterile-filtered solution in PBS, 5 % trehalose
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注意事项
- Avoid repeated freezing and thawing
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储存条件
- 4 °C,-20 °C
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储存方法
- May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.
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- CSPG5 (Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5))
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别名
- CSPG5 / Neuroglycan C
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背景
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Name/Gene ID: CSPG5
Synonyms: CSPG5, CALEB, Neuroglycan C, NGC -
基因ID
- 10675
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UniProt
- O95196
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途径
- Glycosaminoglycan Metabolic Process
抗原
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